TRIM5 antibody orb19133 from Biorbyt

Product Specs
ItemTRIM5 antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb19133
ClonalityPolyclonal
ApplicationsELISA, IHC
Quantity100 µg
HostGoat
Conjugate/TagUnconjugated
TargetTRIM5 / RNF88 (alpha isoform)
Molecular Weight56.3
NCBI Full Gene Nametripartite motif containing 5
NCBI Gene AliasesRNF88, TRIM5alpha
ReactivityHuman
StorageAliquot and store at -20°C. Minimize freezing and thawing

Specifications/Features

Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6ug/ml.WB: Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_149023.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Application Dilutions: ELISA: 1:32000, WB: 0.3 µg/ml, IHC-P: 3-6µg/ml